Centre staff
Dr Bob Smith
Principal lecturer in analytical science
Phone 0114 225 3050
Email r.f.smith@shu.ac.uk
Research interests
Analytical approaches in the assessment of smoking status and its relations to disease
Studies have involved the assessment of smoking status and its relations to diabetes, obesity, pregnancy and antioxidant status. Current work is focussed on the applications of novel derivatisation procedures for HPLC and capillary electrophoresis-mass spectrometry to the measurement of nicotine and its various metabolites.
Recent work has involved the use of Molecularly Imprinted Polymers (MIPS) to create specific binding solid phase absorbents. Insights gained from the study of these analytical techniques are applied to investigating the relationship of nicotine metabolites and disease with consideration of individual nicotine metabolic profiles rather than the measurement of total nicotine exposure. Of particular interest is the use of these techniques, especially CE-MS-MS to the study of the relationship between cytochrome P450 expression and nicotine metabolism in placental cell lines.
Recent publications
Wilson L, Jehanli, A, Hand C, Cooper, G and Smith R.F (2007) Evaluation of a rapid oral fluid point of care test for MDMA Journal of Analytical Toxicology 2007 31: (2) 98-104
Baidoo EEK, Clench MR, Smith RF and Tetler LW (2003). Determination of nicotine and its metabolites in urine by solid-phase extraction and sample stacking capillary electrophoresis-mass spectrometry. Journal of Chromatography B 796 (2): 303-313.
Chambers K, Ellard GA, Hewson A and Smith RF (2001). Improved urine test for the assessment of smoking status. British Journal Biomedical Science 58: 61-65.
Palmer ME, Chambers K, Smith RF and Tetler L (2001). Separation of nicotine metabolites by CZE and CZE/MS Rapid Comm. in Mass.Spec 15: 224-231.
