Centre staff
Dr Dawn Hadden
Senior lecturer in cell biology
Phone 0114 225 3038
Email d.hadden@shu.ac.uk
Research interests
The structure and function of membrane proteins
Function of chlamydia polymorphic membrane proteins
It is thought that these outer membrane proteins may have a role in the pathogenesis of the bacterium. This is currently being investigated in collaboration with Dr A Eley, University of Sheffield Medical School.
Membrane topology of nucleoside transporters
Using the techniques of cysteine scanning mutagenesis and immunocytochemistry, the topology and membrane orientation, together with identification of amino acid residues vital for the function of rat CNT1 and E. coli NupC have been elucidated. This work was undertaken with Professor S Baldwin, University of Leeds.
Interaction of the Arabidopsis protein, PEX19, with peroxisomal membrane proteins
Arabidopsis PEX19 has been shown to bind to peroxisomal membrane proteins (PMP). It is thought to act as a chaperone, shuttling newly synthesised PMPs from the endoplasmic reticulum to the peroxisome membrane. This work was undertaken with Dr A Baker, University of Leeds.
Recent publications
Hadden DA, Phillipson BA, Johnston KA, Brown L-A, Manfield IW, El-Shami M, Sparkes IA, Baker A (2006). Arabidosis PEX19 is a dimeric protein that binds the peroxin PEX10. Molecular Membrane Biology 23(4): 325-326.
Hamilton, S.R., Yao, S.Y.M., Ingram, J.C., Hadden, D.A., Ritzel, M.W., Gallagher, M.P., Henderson, P.J.F., Cass, C.E., Young, J.D. and Baldwin, S.A. (2001) 'Subcellular distribution and membrane topology of the mammalian concentrative Na+-nucleoside cotransporter rCNT1' J. Biol. Chem. 276, 27981-27988.
PhD student
- Louise Vickers - the role of chlamydia trachomatis polymorphic membrane proteins (Pmps) in pathogenicity
