Centre staff
Dr John Bright
Senior lecturer
Phone 0114 225 5237
Email j.bright@shu.ac.uk
Research interests
Characterisation of bacteria by intact cell matrix assisted laser desorption/ionisation mass spectrometry (ICM-MS)
Pioneering research on ICM-MS has led to the commercial development of a rapid method of identifying bacteria firstly by 'Waters / MicroMass and subsequently by Shimadzu / SARAMISTM database and Bruker Daltronics Ltd / Biotyper. These are now been installed in hospitals in GB and on the continent for the rapid identification of bacteria. These commercial systems are able to analyse 1000 isolates a day at a cost of less than £0.01 per sample. Ways of speeding up the identification of bacteria in clinical samples even further and other commercial applications of ICM-MS profiling and imaging are being pursued.
Substratum influences on the physiological activity of bacteria
Surface energy of a substratum can influence the assimilatory and respiratory activity of surface-associated bacteria as well as their degree of adhesion. The overall metabolic efficiency of surface-associated bacteria is greater than for their free-living counterparts. The degree of this metabolic efficiency is dependent on the substratum surface energy. Applications of this research include enhancement of biotechnological processes involving immobilised cells and the prevention of biofouling.
Collaborators
Recent publications
Bright, J. J., Claydon, M. A., Soufian, M. and Gordon, D. B. (2002) Rapid Typing of Bacteria Using Matrix Assisted Laser Desorption Ionisation Time-of-Flight Mass Spectrometry and Pattern Recognition Software. Journal of Microbiological Methods, 48, 127-138.
Bright, J. J., Sutton, H. E., Edwards-Jones, V., Dare, D. J., Keys, C. J., Shah, H. N., McKenna, T., Wells, G. and Lunt, M. (2002). Rapid Identification of Anaerobic Bacteria Using Matrix Assisted Laser Desorption Ionisation Time-of-Flight Mass Spectrometry. The World of Microbes. Paris, France.
Dare, D. J., Bright, J. J., Sutton H. E., Edwards-Jones, V., Keys, C. J., and Shah, H. N., McKenna, T., Lunt, M., Wells, G. (2002). Rapid Identification of Bacteria by Intact Cell Matrix Assisted Laser Desorption/Ionisation Time-of-Flight Mass Spectrometry. 102nd General Meeting of the American Society for Microbiology. Salt Lake City, USA.
Bright, J. J., Sutton, H. E., Edwards-Jones, V., Dare, D. J., Keys, C. J., Shah, H., McKenna, T., Wells, G., and Lunt, M. (2002). Cluster Analysis of Bacteria Based on Data Obtained from Intact Cell Matrix Assisisted Laser Desorption/Ionisation Time-of-Flight Mass Spectrometry. 102nd General Meeting of the American Society for Microbiology. Salt Lake City, USA.
McKenna, T., Lunt, M., Dare, D., Morgan, M., Bright, J. J., Edwards-Jones, V., and Keys, C. (2001) Rapid Identification of Microorganisms by Matrix Assisted Laser Desorption/Ionisation Time-of-Flight Mass Spectrometry: An Inter-Laboratory Study. Abstracts of 49th American Society for Mass Spectrometry, Chicago, Illinois, USA.
Bright, J. J., Dare, D. J., Morgan, M. M., Edwards-Jones, V., Keys, C. J., McKenna T. and Lunt, M. (2001). The Effect of Variations of Sample Preparation on Rapid Identification of Bacteria by Intact Cell Matrix Assisted Laser Desorption/Ionisation Time-of-Flight Mass Spectrometry. 101st General Meeting of the American Society for Microbiology. Florida USA.
Dare, D. J., Bright, J. J., Morgan, M. M., Edwards-Jones, V., McKenna T. and M. Lunt. (2001). Rapid Identification of Bacteria by Intact Cell Matrix Assisted Laser Desorption Time-of-Flight Mass Spectrometry: Inter-laboratory Study. 101st General Meeting of the American Society for Microbiology. Florida USA.
Edwards-Jones, V., Morgan, M. M., Bright, J. J., Dare, D. J., Keys, C. J., Shah, H. N., McKenna, T., and Lunt, M. (2001). Rapid Identification of Bacteria by Matrix Assisted Laser Desorption/Ionisation Mass Spectrometry. Institute for Biomedical Sciences Conference. Birmingham, UK.
McKenna, T., Lunt, M. S., Dare, D. J., Morgan, M. M., Bright, J. J., Edwards-Jones, V., Claydon, M. A,. and Keys, C. J. (2001). The Rapid Identification of Microorganisms by Matrix Assisted Laser Desorption/Ionisation Mass Spectrometry. 101st General Meeting of the American Society for Microbiology. Florida USA.
Bright, J. J., Claydon, M. A., Soufian, M. and Gordon, D. B. (2000) Rapid Typing of Bacteria Using Matrix Assisted Laser Desorption Ionisation Time-of-Flight Mass Spectrometry and Pattern Recognition Software. Abstracts of the IVth International Symposium on the Interface between Analytical Chemistry and Microbiology Tregastel, France. 06.
Bright, J. J., Soufian, M., Claydon, M. A. and Devine, D. A. (2000) Intact Cell Matrix Assisted Laser Desorption Time-of-Flight Mass Spectrometry Analysis of Prevotella intermedia and P. nigrecens Strains. Abstracts of Anaerobe 2000, IXa-O2. 184.
McKenna, T. Claydon, M. A., Bright, J. J., Soufian, M., Evason, D., Edwards-Jones, V., Gordon D. B., Lunt, M. S. and Morris, M. (2000). The Application of Pattern Recognition Software to the Rapid Identification of Microorganisms by Mass Spectrometry. Pittcon 2000. New Orleans USA. 3.
McKenna, T. Lunt, M. S., Morris, M., Claydon, M. A. and Bright, J. J. (2000). The Rapid Identification of Microorganisms by Mass Spectrometry. 48th American Society for Mass Spectrometry. Long Beach, California, USA.
McKenna, T., Lunt, M. S., Cottrell, J., Morris, M., Claydon, M. A., Bright, J. J. and Evason, D. (2000) The Rapid Identification of Microorganisms by Mass Spectrometry. Abstracts of the IVth International Symposium on the Interface between Analytical Chemistry and Microbiology. Tregastel France. P35.
Patel, M., Soufian, M., Bright, J. J., Fletcher-Williams, G, Bunter, J. and Claydon, M. A. (2000). The Analysis of MALDI-ToF-MS Data Obtained from 42 Strains of Peptostreptococci Using Pattern Recognition Software. Anaerobe 2000. Manchester, UK. IXa-P3. 187.
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