Centre staff
Professor Malcolm Clench
Professor of mass spectrometry
Phone 0114 225 3054
Email m.r.clench@shu.ac.uk
Research interests
Biomedical and environmental mass spectrometry
Mass spectrometry (MS) is used to solve a range of bioanalytical problems. In particular a range of projects are in progress which are concerned with the utilisation of matrix assisted laser desorption mass spectrometry imaging (MALDI-MSI) for imaging the distribution of xenobiotics and endogenous compounds in biological tissue.
Development of new techniques in mass spectrometry
For example imaging MALDI-MS. This development work is carried out in collaboration with Waters Corporation, Manchester UK and Elforlight Lid, Daventry UK and has led to one patent on improvements in MALDI sensitivity.
Development of mass spectrometric methods for the detection of xenobiotics and endogenous compounds in biological tissue.
Imaging MS can be use to study the distribution of non-radiolabelled compounds within biological tissue. This offers significant advantages over conventional methods of analysis and can be used to study for example the distribution of anti-cancer drugs in tumours and to monitor the development of fungal infections. This work is carried out in collaboration with Dr Vikki Carolan, Dr Simona Francese (BMRC), Professor Mike Burrell (University of Sheffield), Dr Peter Marshall, Josie Morrell (GlaxoSmithKline), Dr Don Richards (Pfizer Global R and D), Dr Paul Loadman (Institute of Cancer Therapeutics, University of Bradford), Dr Andrew McEwen, (Quotient Bioresearch).
Application of MALDI-MSI to pharmaco/toxicodynamic responses
Currently projects are investigating changes in protein and lipid expression when skin is exposed to sensitizing chemicals and the response of tumours to treatment with vascular disrupting agents (VDA) by this approach.
Current work is supported by grants and gifts of equipment from MRC/GSK, CRUK/EPSRC, The British Mass Spectrometry Society, Syngenta and Pfizer Global R&D.
Collaborators
- Dr Susan Crosland, Dr Kate Sharples: Syngenta
- Dr Paul Loadman,Dr Chris Sutton: University of Bradford
- Dr Peter Marshall, Josie Morrell: GSK
- Peter Scriven, The University of Sheffield
- Professor Gillian Tozer, Professor Martyn Paely, The University of Sheffield
- Keith Oakes, Elforlight Ltd, Daventry UK.
- Emmanuel Claude, Waters Corporation, Manchester UK.
- Professor Elaine Holmes, Imperial College, London UK
Recent publications
Trim P.J., Djidja M-C., Atkinson S.J., Oakes K, Cole L.M., Anderson D.M.G., Hart P.J, Francese S and Clench M.R. Analytical and Bioanalytical Chemistry 397 (2010) 3409-3419 'A Hybrid Quadrupole Time of Flight Mass Spectrometer Modified to Incorporate a 20kHz Nd:YVO4 Laser for MALDI-MS Imaging'.
Anderson D.M.G., Carolan V.A., Crosland S, Sharples K.R. and Clench M.R. Rapid Communications in Mass Spectrometry 24 (2010) 3309-3319 'Examination of the Translocation of Sulfonylurea Herbicides in Sunflower Plants by MALDI-MSI'
Djidja M-C., Claude E, Snell M, Scriven P., Carolan V.A. and Clench M.R. Analytical and Bioanalytical Chemistry 397 (2010) 587-601 'Novel Molecular Tumour Classification using MALDI-Mass Spectrometry Imaging of Tissue Micro Array'.
Earnshaw C.J., Carolan V.A., Richards D.S. and Clench M.R. Rapid Communications in Mass Spectrometry 24(2010)1665-1672 'Direct Analysis of Tablet Formulations Using Matrix Assisted Laser Desorption Mass Spectrometry Imaging.'
Djidja M-C., Francese S., Loadman P.M. Sutton C., Scriven P., Claude E, Snell M, Franck J., Salzet M. and Clench M.R Proteomics 9 (2009) 2750-2763 'Detergent addition to tryptic digests and Ion Mobility separation prior to MS/MS improves peptide yield and Protein Identification for in situ Proteomic Investigation of Frozen and FFPE Adenocarcinoma tissue sections.'
Wostenholme R., Bradshaw R., Clench M.R. and Francese S. Rapid Communications in Mass Spectrometry 23 (2009)3031-3039 'Study of Latent Fingermarks by MALDI MS Imaging of endogenous lipids'
Djidja M-C., Claude E, Snell M, Scriven P., Francese S., Carolan V.A. and Clench M.R Journal of Proteome Research 8(2009)4876-4884 'MALDI-Ion Mobility Separation-Mass Spectrometry Imaging of Glucose-Regulated Protein 78 kDa (Grp78) in Human Formalin Fixed Paraffin Embedded Pancreatic Adenocarcinoma Tissue Sections'
Denney H, Clench M.R. and Woodroofe M.N., Biochemical and Biophysical Research Communications 382 (2009) 341-347 'Cleavage of chemokines CCL2 and CXCL10 by matrix metalloproteinases-2 and -9: implications for chemotaxis'.
Trim P.J., Francese S. and Clench M.R. Bioanalysis 1 (2009) 309-319 'Imaging Mass Spectrometry for the Assessment of Drugs and Metabolites in Tissue'.
Zhou Y, Taylor B., Smith TJ, Liu Z-P, Clench M, Davies, N Rainsford KD., Journal of Pharmacy and Pharmacology (2009) (In press) 'A novel compound from celery seed with a bactericidal effect against Helicobacter pylori'
Anderson DMG, Carolan VA, Crosland S, Sharples KR and Clench MR. Rapid Communications in Mass Spectrometry 23(2009)1321-1327 'Examination of the Distribution of Nicosulfuron in Sunflower Plants by MALDI-MSI'
Djidja M-C., Carolan V.A., Loadman P.M. and Clench M.R. Rapid Communications in Mass Spectrometry 22(2008)1615-1618 'Method Development for Protein Profiling in Biological Tissues by MALDI-Mass Spectrometry Imaging'
Trim P.J,, Henson C, Avery JL, McEwen A, Snel MF, Claude E, Marshall PM, West A, Princivalle AP and Clench MR. Analytical Chemistry 80 (2008) 8628-8634 'Matrix Assisted Laser Desorption Ionisation - Ion Mobility Separation - Mass Spectrometry Imaging of Vinblastine in Whole Body Tissue Sections'.
Trim P.J., Atkinson S.J., Princivalle A.P., Marshall P.S., West A. and Clench M.R. Rapid Communications in Mass Spectrometry 22 (2008) 1503-1509 'MALDI-MS Imaging of Lipids in Rat Brain Tissue with Integrated Unsupervised and Supervised Multivariant Statistical Analysis'.
Newton K.A., Clench M.R., Deshmukh R., Jeyaseelan K. and Strong P.N. Rapid Communications in Mass Spectrometry 21(2007) 3467-3476 'Mass Fingerprinting of toxic fractions from the venom of the Indian red scoprion, Mesobuthus tamulus: biotope-specific variation in the expression of venom peptides'.
Prideaux B, Atkinson S.J., Carolan V.A., Morton J and Clench M.R., International Journal of Mass Spectrometry, 260 (2007) 243-251 'Sample Preparation and Data Interpretation Procedures for the Examination of Xenobiotic Compounds in Skin by Indirect Imaging MALDI-MS'.
Atkinson S.J., Loadman P.M., Sutton C., Patterson L.H. and Clench M.R., Rapid Communications in Mass Spectrometry 21 (2007) 1271-1276 'Examination of the Distribution of the Bioreductive Drug AQ4N and its Active Metabolite AQ4 in Solid Tumours by Imaging Matrix Assisted Laser Desorption Ionisation Mass Spectrometry'.
Robinson S., Warburton K., Seymour M., Clench M. and Thomas-Oates J. New Phytologist 173 (2007) 438-444. 'A Study of the Localisation of Water Soluble Carbohydrates in Wheat Stems Using Imaging Matrix Assisted Laser Desorption Ionisation Mass Spectrometry'.
Burrell M., Earnshaw C. and Clench M.R. Journal of Experimental Botany 58(2007) 757-763 'Imaging MALDI-MS - A technique to map plant metabolites within tissues at high spatial resolution'.
Grants held
2009-2012, MRC CASE Award (GlaxoSmithKline) ' The application of MALDI-Mass Spectrometry and related techniques for imaging drug disposition in asthma, COPD and other respiratory disease models following inhaled delivery'
2008-2013, CRUK/EPSRC 'Development of new techniques in magnetic resonance and spectroscopy imaging (MRI and MRSI) and matrix-assisted laser desorption ionization mass spectrometry imaging (MALDI-MSI), as bio-markers for clinical development of tumour vascular-targeting agents'(with Prof Gillian Tozer/Prof Martyn Paley, University of Sheffield)
PhD students
- Huda Harati - determination of trace pollutants in produced water from Lybian oil fields
- Philippa Hart - Direct tissue profiling and imaging by mass spectrometry as a novel method for the investigation
- Laura Cole - Magnetic resonance and matrix-assisted laser desorption mass spectrometry imaging for progressing the development of tumour vascular targeted drugs.
- Bryn Flinders - Magnetic resonance and matrix-assisted laser desorption mass spectrometry imaging for progressing the development of tumour vascular targeted drugs.
- Scott Martin - Mass spectrometry of reactive metabolites.
- Salem Omar - A study of bioremediation relevant to the petroleum industry using methanotrphs and other microorganisms
- Afnan Batubara - Studies into the distribution of anti-ancer drugs in solid tumours by Mass Spectrometry
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