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Professor Tom Smith BSc, PhD

Professor of Microbiology


Research

Bacterial oxygenases

Bacteria that can grow using hydrocarbons as their sole source of carbon and energy produce oxygenases that have the ability to perform specific oxygenation of a wide range of hydrocarbons and chlorinated organic compounds, many of which are unreactive towards oxidation by chemical means. Bacterial oxygenases are thus potentially useful in bioremediation and, thanks to the remarkable stereo- and regio-selectivity that such enzymes can demonstrate, have potential applications in the synthesis of fine chemicals.

Soluble methane monooxygenase (sMMO) from methane-oxidising bacteria naturally oxidises methane to methanol, which is the first step in biological oxidation of the potent greenhouse gas methane. It also co-oxidises a wide range of hydrocarbon and chlorinated hydrocarbon substrates. We are using mutagenesis-based methods to investigate and manipulate the very wide substrate range of sMMO and to investigate the mechanism of oxidation of the enzyme's kinetically unreactive substrate methane. This work is currently funded by a grant from the Biotechnology and Biological Sciences Research Council (BBSRC) and is a collaboration with Professor J Colin Murrell at the University of East Anglia. We are also working with Professor John Lipscomb (University of Minnesota) to study reaction kinetics in sMMO.

We are performing structure-function studies on alkene monooxygenase (AMO), which is produced by the alkene-oxidising bacterium Rhodococcus rhodochrous B-276 and can be used to produce high-value chiral epoxides.

New expression systems for biocatalysts

In addition to the expression systems that have already been developed for AMO and sMMO, work is also underway exploring the expression of stereoselective oxygenases in a bacterium that grows using methanol as its carbon and energy source in order to produce high-value biocatalysts using methanol as the feedstock. This project is in collaboration with Dr C Míguez and co-workers at the Biotechnology Research Institute, Montreal.

Molecular environmental microbiology of the hospital ward environment

We are applying molecular microbial ecology methods, including the polymerase chain reaction (PCR) and denaturing gradient gel electrophoresis (DGGE) to study populations of microorganisms and resistance genes within the internal environment of hospital wards and other areas, as a new tool to study the role of the environment in hospital-acquired infections. This work is a collaboration with Dr Karen Stanley (Sheffield Hallam) and Mr Robert Kerry and Dr Robert Townsend (Sheffield Teaching Hospitals NHS Foundation Trust). It is funded by the Hospital Infection Society.

Microbiological applications in materials science

Research is underway into applications of bacteria within materials science, including inhibition of corrosion and biofouling and development of biosensors. This work is in collaboration with Professor Bob Akid at the University of Manchester. Work on development of a novel coating containing 'good' bacteria for inhibition of biofouling and corrosion is currently supported by the Engineering and Physical Sciences Research Council (EPSRC).

Novel antimicrobial systems

Work is under way to develop a new controlled-release coating for local delivery of antibiotics and other therapeutic agents for use on orthopedic prostheses and other surgical implants. This work is a collaboration with Professor Bob Akid at the University of Manchester and Dr Robert Townsend and Professor Ian Stockley (Sheffield Teaching Hospitals NHS trust).

We are also working on isolation and characterisation of antimicrobial agents from natural products, including celery seeds. This work is a collaboration with Professor K D Rainsford (Sheffield Hallam University) and Dr Graham Stafford (School of Clinical Dentistry, University of Sheffield) and Dr Simon Jones (Department of Chemistry, University of Sheffield).

Bioremediation of heavy metal and organic pollutants

Microorganisms can be exploited to mitigate environmental pollution, by oxidising organic pollutants (such as hydrocarbons and chlorinated hydrocarbons) and by changing the speciation of heavy metals to make them less bioavailable. Work in collaboration with Dr Philip Gardiner investigates the potential of methane-oxidising and other environmental bacteria to remediate hexavalent chromium contamination. Other work is concerned with bioremediation of wastes from the petroleum industry.

Publications

Journal articles

Karthikeyan, O.P., Smith, T.J., Dandare, S.U., Parwin, K.S., Singh, H., Loh, H.X., ... Kumaresan, D. (2021). Metal(loid) speciation and transformation by aerobic methanotrophs. Microbiome, 9 (1). http://doi.org/10.1186/s40168-021-01112-y

Nichol, T., Callaghan, J., Townsend, R., Stockley, I., Hatton, P.V., Le Maitre, C., ... Akid, R. (2021). The antimicrobial activity and biocompatibility of a controlled gentamicin-releasing single-layer sol-gel coating on hydroxyapatite-coated titanium. The Bone & Joint Journal, 103-B (3), 522-529. http://doi.org/10.1302/0301-620x.103b3.bjj-2020-0347.r1

Henly, E.L., Norris, K., Rawson, K., Zoulias, N., Jaques, L., Chirila, P.G., ... Forbes, S. (2021). Impact of long-term quorum sensing inhibition on uropathogenic Escherichia coli. Journal of Antimicrobial Chemotherapy. http://doi.org/10.1093/jac/dkaa517

Hufton, J., Harding, J., Smith, T., & Romero-González, M.E. (2020). The importance of the bacterial cell wall in uranium(VI) biosorption. Phys Chem Chem Phys. http://doi.org/10.1039/d0cp04067c

Enbaia, S., Eswayah, A., Hondow, N., Gardiner, P., & Smith, T. (2020). Detoxification, active uptake, and intracellular accumulation of chromium species by a methane-oxidizing bacterium. Applied and environmental microbiology. http://doi.org/10.1128/AEM.00947-20

Challa, S., & Smith, T. (2020). Isolation of a methane-oxidizing bacterium that bioremediates hexavalent chromium from a formerly industrialized Suburban River. Letters In Applied Microbiology. http://doi.org/10.1111/lam.13330

Eswayah, A.S., Hondow, N., Scheinost, A.C., Merroun, M., Romero-González, M., Smith, T.J., & Gardiner, P.H.E. (2019). Methyl Selenol as Precursor in Selenite Reduction to Se/S Species by Methane-oxidizing Bacteria. Appl Environ Microbiol. http://doi.org/10.1128/AEM.01379-19

Codjoe, F.S., Brown, C.A., Smith, T., Miller, K., & Donkor, E.S. (2019). Genetic relatedness in carbapenem-resistant isolates from clinical specimens in Ghana using ERIC-PCR technique. PLoS One, 14 (9), e0222168. http://doi.org/10.1371/journal.pone.0222168

Codjoe, F.S., Donkor, E.S., Smith, T., & Miller, K. (2019). Phenotypic and genotypic characterization of carbapenem-resistant gram-negative bacilli pathogens from hospitals in Ghana. Microbial drug resist. http://doi.org/10.1089/mdr.2018.0278

Abu-Ali, H., Nabok, A., & Smith, T.J. (2019). Development of novel and highly specific ssDNA-Aptamer-based electrochemical biosensor for rapid detection of Mercury (II) and Lead (II) ions in water. Chemosensors, 7 (2), e27. http://doi.org/10.3390/chemosensors7020027

Abu-Ali, H., Nabok, A., & Smith, T. (2019). Electrochemical inhibition bacterial sensor array for detection of water pollutants: artificial neural network (ANN) approach. Analytical and Bioanalytical Chemistry. http://doi.org/10.1007/s00216-019-01853-8

Pakiet, M., Kowalczyk, I., Leiva Garcia, R., Moorcroft, R., Nichol, T., Smith, T., ... Brycki, B. (2019). Gemini surfactant as multifunctional corrosion and biocorrosion inhibitors for mild steel. Bioelectrochemistry, 128, 252-262. http://doi.org/10.1016/j.bioelechem.2019.04.005

Herrero‐Hernandez, E., Greenfield, D., Smith, T., & Akid, R. (2019). Evaluation of the performance of a mediatorless microbial fuel cell by electrochemical impedance spectroscopy. Electroanalysis. http://doi.org/10.1002/elan.201900120

Abu-Ali, H., Nabok, A., Smith, T., & Al-Shanawa, M. (2019). Development of a novel electrochemical inhibition sensor array based on bacteria immobilized on modified screen-printed gold electrodes for water pollution detection. BioNanoScience, 9 (2), 345-355. http://doi.org/10.1007/s12668-019-00619-x

Henly, E., Dowling, J., Maingay, J.B., Lacey, M., Smith, T., & Forbes, S. (2019). Biocide exposure induces changes in susceptibility, pathogenicity and biofilm formation in Uropathogenic Escherichia coli. Antimicrobial agents and chemothery. http://doi.org/10.1128/AAC.01892-18

Eyice, Ö., Myronova, N., Pol, A., Carrión, O., Todd, J.D., Smith, T., ... Schäfer, H. (2018). Bacterial SBP56 identified as a Cu-dependent methanethiol oxidase widely distributed in the biosphere. The ISME journal, 1 (12), 145-160. http://doi.org/10.1038/ismej.2017.148

Abu-Ali, H.F., Nabok, A., Smith, T., & Al-Shanawa, M.A. (2018). Electrochemical inhibition biosensor array for rapid detection of water pollutions based on bacteria immobilized on screen-printed gold electrodes. European Chemical Bulletin, 7 (10), 307-314. http://doi.org/10.17628/ecb.2018.7.307-314

Suleiman, R., Gittens, J., Khaled, M., Smith, T.J., Akid, R., El Ali, B., & Khalil, A. (2017). Assessing the Anticorrosion and Antifouling Performances of a Sol–Gel Coating Mixed with Corrosion Inhibitors and Immobilised Bacterial Endospores. Arabian Journal for Science and Engineering, 42 (10), 4327-4338. http://doi.org/10.1007/s13369-017-2419-0

Harrison, P., Harrison, T., Stockley, I., & Smith, T. (2017). Does tantalum exhibit any intrinsic antimicrobial or antibiofilm properties? The bone & joint journal, 99-B (9), 1153-1156. http://doi.org/10.1302/0301-620X.99B9.BJJ-2016-1309.R1

Lock, M., Nichol, T., Murrell, J.C., & Smith, T. (2017). Mutagenesis and expression of methane monooxygenase to alter regioselectivity with aromatic substrates. FEMS microbiology letters, 364 (13), fnx137. http://doi.org/10.1093/femsle/fnx137

Eswayah, A., Smith, T., Scheinost, A., Hondow, N., & Gardiner, P. (2017). Microbial transformations of selenite by methane-oxidizing bacteria. Applied Microbiology and Biotechnology, 101 (17), 6713-6724. http://doi.org/10.1007/s00253-017-8380-8

Andrews, A.M., Haywood-Small, S., Smith, T., & Stafford, P. (2017). Interactions of periodontal pathogens with megakaryocytic cells and platelets. Journal of Oral Microbiology, 9 (sup1), 1325245. http://doi.org/10.1080/20002297.2017.1325245

Frew, N.M., Cannon, T., Nichol, T., Smith, T., & Stockley, I. (2017). Comparison of the elution properties of commercially available gentamicin and bone cement containing vancomycin with 'home-made' preparations. The bone & joint journal, 98-B (1), 73-77. http://doi.org/10.1302/0301-620X.99B1.BJJ-2016-0566.R1

Abu-Ali, H., Nabok, A., Smith, T., & Al-Shanawa, M. (2016). Development of electrochemical inhibition biosensor based on bacteria for detection of environmental pollutants. Sensing and Bio-Sensing Research, 13, 109-114. http://doi.org/10.1016/j.sbsr.2016.10.007

Nichol, T., Smith, T., Townsend, R., Stockley, I., & Akid, R. (2016). Analysis of linezolid and tigecycline as candidates for local prophylaxis via antibiotic-loaded bone cement. The Journal of antimicrobial chemotherapy, 72 (2), 410-416. http://doi.org/10.1093/jac/dkw410

Eswayah, A., Smith, T., & Gardiner, P. (2016). Microbial transformations of selenium species of relevance to bioremediation. Applied and environmental microbiology, 82 (16), 4848-4859. http://doi.org/10.1128/AEM.00877-16

Cao, S.-.L., Yue, D.-.M., Li, X.-.H., Smith, T., Li, N., Zong, M.-.H., ... Lou, W.-.Y. (2016). Novel nano-/micro-biocatalyst: soybean epoxide hydrolase immobilized on UiO-66-NH2 MOF for efficient biosynthesis of enantiopure (R)-1, 2-octanediol in deep eutectic solvents. ACS Sustainable Chemistry and Engineering, 4 (6), 3586-3595. http://doi.org/10.1021/acssuschemeng.6b00777

Deng, X., Cao, S., Li, N., Wu, H., Smith, T., Zong, M., & Lou, W. (2016). A magnetic biocatalyst based on mussel-inspired polydopamine and its acylation of dihydromyricetin. Chinese Journal of Catalysis, 37 (4), 584-595. http://doi.org/10.1016/S1872-2067(15)61045-2

Salih, S., Paskins, A., Nichol, T., Smith, T., & Hamer, A. (2015). The cement spacer with multiple indentations : increasing antibiotic elution using a cement spacer 'teabag'. The Bone & Joint Journal, 97-B (11), 1519-1524. http://doi.org/10.1302/0301-620X.97B11.35618

Eduok, U., Suleiman, R., Gittens, J., Khaled, M., Smith, T.J., Akid, R., ... Khalil, A. (2015). Anticorrosion/antifouling properties of bacterial spore-loaded sol-gel type coating for mild steel in saline marine condition: a case of thermophilic strain of Bacillus licheniformis. RSC Advances, 5 (114), 93818-93830. http://doi.org/10.1039/C5RA16494J

Nichol, T., Murrell, J.C., & Smith, T.J. (2015). Controlling the activities of the Diiron Centre in bacterial monooxygenases: lessons from mutagenesis and biodiversity. European Journal of Inorganic Chemistry, 2015 (21), 3419-3431. http://doi.org/10.1002/ejic.201500043

Sigdel, S., Hui, G., Smith, T.J., Murrell, J.C., & Lee, J.-.K. (2015). Molecular dynamics simulation to rationalize regioselective hydroxylation of aromatic substrates by soluble methane monooxygenase. Bioorganic & Medicinal Chemistry Letters, 25 (7), 1611-1615. http://doi.org/10.1016/j.bmcl.2015.01.069

Barker, S., Nichol, T., Harrison, P.L., Stockley, I., Townsend, R., & Smith, T.J. (2015). Temocillin: a new candidate antibiotic for local antimicrobial delivery in orthopaedic surgery? The Journal of antimicrobial chemotherapy, 70 (3), 780-783. http://doi.org/10.1093/jac/dku425

Al-Shanawa, M., Nabok, A., Hashim, A., Smith, T., & Forder, S. (2014). Optical study of the effect of gamma radiation and heavy metals on microorganisms (Bacteria). BioNanoScience, 4 (2), 180-188. http://doi.org/10.1007/s12668-014-0133-x

Suleiman, R., Khaled, M., Wang, H., Smith, T.J., Gittens, J., Akid, R., ... Khalil, A. (2014). Comparison of selected inhibitor doped sol–gel coating systems for protection of mild steel. Corrosion engineering science and technology, 49 (3), 189-196. http://doi.org/10.1179/1743278213Y.0000000113

Sow, S.L.S., Khoo, G., Chong, L.K., Smith, T.J., Harrison, P.L., & Ong, H.K.A. (2014). Molecular diversity of the methanotrophic bacteria communities associated with disused tin-mining ponds in Kampar, Perak, Malaysia. World Journal of Microbiology and Biotechnology, 30 (10), 2645-2653. http://doi.org/10.1007/s11274-014-1687-z

Hu, S., Smith, T., Lou, W., & Zong, M. (2014). Efficient hydrolysis of cellulose over a novel sucralose-derived solid acid with cellulose-binding and catalytic sites. Journal of Agricultural and Food Chemistry, 62 (8), 1905-1911. http://doi.org/10.1021/jf405712b

Sow, S.L.S., Khoo, G., Chong, L.K., Smith, T.J., Harrison, P.L., & Ong, H.K.A. (2014). Molecular diversity of the ammonia-oxidizing bacteria community in disused tin-mining ponds located within Kampar, Perak, Malaysia. World Journal of Microbiology and Biotechnology, 30 (2), 757-766. http://doi.org/10.1007/s11274-013-1506-y

Herrero-Hernandez, E., Smith, T., & Akid, R. (2013). Electricity generation from wastewaters with starch as carbon source using a mediatorless microbial fuel cell. Biosensors and Bioelectronics, 39 (1), 194-198. http://doi.org/10.1016/j.bios.2012.07.037

Gittens, J.E., Smith, T.J., Suleiman, R., & Akid, R. (2013). Current and emerging environmentally-friendly systems for fouling control in the marine environment. Biotechnology Advances, 31 (8), 1738-1753. http://doi.org/10.1016/j.biotechadv.2013.09.002

Bansal, V., Hou, X.D., Liu, Q.P., Smith, T., Li, N., & Zong, M.H. (2013). Evaluation of toxicity and biodegradability of cholinium amino acids ionic liquids. PLoS ONE, 8 (3), e59145. http://doi.org/10.1371/journal.pone.0059145

Loh, J.Y., Ong, H.K.A., Hii, Y.S., Smith, T.J., Lock, M.M., & Khoo, G. (2013). Impact of potential food sources on the life table of the cladoceran, Moina macrocopa. Israeli Journal of Aquaculture - Bamidgeh, 65 (1).

Al-Shanawa, M., Nabok, A., Hashim, A., & Smith, T. (2013). Detection of ionization radiation effect using microorganism (Escherichia Coli). Sensors and Transducers, 149 (2), 179-186.

Chen, W.J., Lou, W.Y., Yu, C.Y., Wu, H., Zong, M.H., & Smith, T. (2012). Use of hydrophilic ionic liquids in a two-phase system to improve Mung bean epoxide hydrolases-mediated asymmetric hydrolysis of styrene oxide. Journal of Biotechnology, 162 (2-3), 183-190. http://doi.org/10.1016/j.jbiotec.2012.09.006

Loh, J.Y., Ong, H.K.A., Yii, Y.S., Smith, T., Lock, M.W., & Khoo, G. (2012). Highly Unsaturated Fatty Acid (HUFA) retention in the freshwater Cladoceran, Moina macrocopa, entriched with lipid emulsions. Israeli Journal of Aquaculture, 64, 637-645. http://www.siamb.org.il/articles-1180-The-Israeli-Journal-of-Aquaculture-Bamidgeh-(IJA).aspx

Hou, X.D., Smith, T., Li, N., & Zong, M.H. (2012). Novel renewable ionic liquids as highly effective solvents for pretreatment of rice straw biomass by selective removal of lignin. Biotechnology and Bioengineering, 109 (10), 2484-2493. http://doi.org/10.1002/bit.24522

Huang, C., Wu, H., Smith, T., Liu, Z., Lou, W.Y., & Zong, M. (2012). In vivo detoxification of furfural during lipid production by the oleaginous yeast Trichosporon fermentans. Biotechnology Letters, 34 (9), 1637-1642. http://doi.org/10.1007/s10529-012-0948-x

Chen, X.H., Wang, X.T., Lou, W.Y., Li, Y., Wu, H., Zong, M.H., ... Chen, X.D. (2012). Immobilization of Acetobacter sp. CCTCC M209061 for efficient asymmetric reduction of ketones and biocatalyst recycling. Microbial Cell Factories, 11. http://doi.org/10.1186/1475-2859-11-119

Lou, W.-.Y., Guo, Q., Chen, W.-.J., Zong, M.-.H., Wu, H., & Smith, T. (2012). A highly active bagasse-derived solid acid catalyst with properties suitable for production of biodiesel. ChemSusChem, 5 (8), 1533-1541. http://doi.org/10.1002/cssc.201100811

Dodds, S., Smith, T.S., Akid, R., Stephenson, J., Nichol, T., Banerjee, R.D., ... Townsend, R. (2011). Contrasting effects of physical wear on elution of two antibiotics from orthopaedic cement. Antimicrobial Agents and Chemotherapy, 56 (3), 1471-1475. http://doi.org/10.1128/AAC.01588-10

Chen, X.-.H., Lou, W.-.Y., Zong, M.H., & Smith, T.S. (2011). Optimization of culture conditions to produce high yields of active Acetobacter sp. CCTCC M209061 cells for anti-Prelog reduction of prochiral ketones. BMC Biotechnology, 11, 110. http://doi.org/10.1186/1472-6750-11-110

Akid, R., Wang, H., Gobara, M., Smith, T.J., & Gittens, J. (2011). Green coatings for industrial applications. Corrosion Management, (100), 11-14.

Smith, T.J., & Murrell, J.C. (2011). Mutagenesis of soluble methane monooxygenase. . http://doi.org/10.1016/B978-0-12-386905-0.00009-7

Al Hasin, A., Gurman, S.J., Murphy, L.M., Perry, A., Smith, T.J., & Gardiner, P.H.E. (2010). Remediation of chromium(VI) by a methane-oxidizing bacterium. Environmental science & technology, 44 (1), 400-405. http://doi.org/10.1021/es901723c

Jiang, H., Chen, Y., Jiang, P.X., Zhang, C., Smith, T.J., Murrell, J.C., & Xing, X.H. (2010). Methanotrophs: Multifunctional bacteria with promising applications in environmental bioengineering. Biochemical Engineering Journal, 49 (3), 277-288. http://doi.org/10.1016/j.bej.2010.01.003

Li, N., Smith, T.J., & Zong, M.H. (2010). Biocatalytic transformation of nucleoside derivatives. Biotechnology Advances, 28 (3), 348-366. http://doi.org/10.1016/j.biotechadv.2010.01.006

Yang, R.-.L., Li, N., Li, R.-.F., Smith, T.J., & Zong, M.-.H. (2010). A highly regioselective route to arbutin esters by immobilized lipase from Penicillium expansum. Bioresource technology, 101 (1), 1-5. http://doi.org/10.1016/j.biortech.2009.07.067

Lou, W.-.Y., Wang, W., Smith, T.J., & Zong, M.-.H. (2009). Biocatalytic anti-Prelog stereoselective reduction of 4′-methoxyacetophenone to (R)-1-(4-methoxyphenyl)ethanol with immobilized Trigonopsis variabilis AS2.1611 cells using an ionic liquid-containing medium. Green chemistry, 11 (9), 1377-1384. http://doi.org/10.1039/b823502c

Murrell, J.C., & Smith, T.J. (2009). Microbial biotechnology meets environmental microbiology. Microbial biotechnology, 2 (2), 142-143. http://doi.org/10.1111/j.1751-7915.2009.00090_11.x

Lou, W.-.Y., Chen, L., Zhang, B.-.B., Smith, T.J., & Zong, M.-.H. (2009). Using a water-immiscible ionic liquid to improve asymmetric reduction of 4-(trimethylsilyl)-3-butyn-2-one catalyzed by immobilized Candida parapsilosis CCTCC M203011 cells. BMC biotechnology, 9 (90). http://doi.org/10.1186/1472-6750-9-90

Zhou, Y., Taylor, B., Smith, T.J., Liu, Z.-.P., Clench, M., Davies, N.W., & Rainsford, K.D. (2009). A novel compound from celery seed with a bactericidal effect against Helicobacter pylori. Journal of pharmacy and pharmacology, 61 (8), 1067-1077. http://doi.org/10.1211/jpp/61.08.0011

Akid, R., Wang, H., Smith, T.J., Greenfield, D., & Earthman, J.C. (2008). Biological functionalization of a sol-gel coating for the mitigation of microbial-induced corrosion. Advanced functional materials, 18 (2), 203-211. http://doi.org/10.1002/adfm.200600493

Borodina, E., Nichol, T., Dumont, M.G., Smith, T.J., & Murrell, J.C. (2007). Mutagenesis of the "Leucine Gate" to explore the basis of catalytic versatility in soluble methane monooxygenase. Applied and environmental microbiology, 73 (20), 6460-6467. http://doi.org/10.1128/AEM.00823-07

Zong, M.H., Duan, Z.Q., Lou, W.Y., Smith, T.J., & Wu, H. (2007). Preparation of a sugar catalyst and its use for highly efficient production of biodiesel. Green Chemistry, 9 (5), 434-443. http://doi.org/10.1039/b615447f

Li, X.F., Lou, W.Y., Smith, T.J., Zong, M.H., Wu, H., & Wang, J.F. (2006). Efficient regioselective acylation of 1-β-D-arabinofuranosylcytosine catalyzed by lipase in ionic liquid containing systems. Green Chemistry, 8 (6), 538-544. http://doi.org/10.1039/b600397d

Lou, W.Y., Zong, M.H., Smith, T.J., Wu, H., & Wang, J.F. (2006). Impact of ionic liquids on papain: An investigation of structure–function relationships. Green Chemistry, 8 (6), 509-512. http://doi.org/10.1039/b600930a

Perry, A., & Smith, T.J. (2006). Protocol for mutagenesis of alkene monooxygenase and screening for modified enantiocomposition of the epoxypropane product. Journal of biomolecular screening, 11 (5), 553-556. http://doi.org/10.1177/1087057106287930

Chongcharoen, R., Smith, T.J., Flint, K.P., & Dalton, H. (2005). Adaptation and acclimatization to formaldehyde in methylotrophs capable of high-concentration formaldehyde detoxification. Microbiology, 151 (8), 2615-2622. http://doi.org/10.1099/mic.0.27912-0

Fosdike, W.L.J., Smith, T.J., & Dalton, H. (2005). Adventitious reactions of alkene monooxygenase reveal common reaction pathways and component interactions among bacterial hydrocarbon oxygenases. FEBS Journal, 272 (11), 2661-2669. http://doi.org/10.1111/j.1742-4658.2005.04675.x

Adeosun, E.K., Smith, T.J., Hoberg, A.M., Velarde, G., Ford, R., & Dalton, H. (2004). Formaldehyde dehydrogenase preparations from Methylococcus capsulatus (Bath) comprise methanol dehydrogenase and methylene tetrahydromethanopterin dehydrogenase. Microbiology, 150 (3), 707-713. http://doi.org/10.1099/mic.0.26707-0

Astier, Y., Balendra, S., Hill, H.A.O., Smith, T.J., & Dalton, H. (2003). Cofactor-independent oxygenation reactions catalyzed by soluble methane monooxygenase at the surface of a modified gold electrode. European Journal of Biochemistry, 270 (3), 539-544. http://doi.org/10.1046/j.1432-1033.2003.03411.x

Smith, T.J., Slade, S.E., Burton, N.P., Murrell, J.C., & Dalton, H. (2002). Improved system for protein engineering of the hydroxylase component of soluble methane monooxygenase. Applied and Environmental Microbiology, 68 (11), 5265-5273. http://aem.asm.org/cgi/content/abstract/68/11/5265

Callaghan, A.J., Smith, T.J., Slade, S.E., & Dalton, H. (2002). Residues near the N-terminus of protein B control autocatalytic proteolysis and the activity of soluble methane mono-oxygenase. European Journal of Biochemistry, 269 (7), 1835-1843. http://doi.org/10.1046/j.1432-1033.2002.02829.x

Balendra, S., Lesieur, C., Smith, T.J., & Dalton, H. (2001). Positively charged amino acids are essential for electron transfer and protein-protein interactions in the soluble methane monooxygenase complex from methylococcus capsulatus (Bath). Biochemistry, 41 (8), 2571-2579. http://doi.org/10.1021/bi015714g

Smith, T.J., Blackman, S.A., & Foster, S.J. (2000). Autolysins of Bacillus subtilis: multiple enzymes with multiple functions. MICROBIOLOGY-UK, 146, 249-262. http://doi.org/10.1099/00221287-146-2-249

Smith, T.J., Lloyd, J.S., Gallagher, S.C., Fosdike, W.L.J., Murrell, J.C., & Dalton, H. (1999). Heterologous expression of alkene monooxygenase from Rhodococcus rhodochrous B-276. European Journal of Biochemistry, 260 (2), 446-452. http://doi.org/10.1046/j.1432-1327.1999.00179.x

Smith, T.J., & Foster, S.J. (1997). Autolysins during sporulation of Bacillus subtilis 168. FEMS MICROBIOLOGY LETTERS, 157 (1), 141-147. http://doi.org/10.1111/j.1574-6968.1997.tb12765.x

Smith, T.J., Blackman, S.A., & Foster, S.J. (1996). Peptidoglycan hydrolases of Bacillus subtilis 168. MICROBIAL DRUG RESISTANCE-MECHANISMS EPIDEMIOLOGY AND DISEASE, 2 (1), 113-118. http://doi.org/10.1089/mdr.1996.2.113

Chen, C.C., Smith, T.J., Kapadia, G., Wasch, S., Zawadzke, L.E., Coulson, A., & Herzberg, O. (1996). Structure and kinetics of the beta-lactamase, mutants S70A and K73H from Staphylococcus aureus PC1. BIOCHEMISTRY, 35 (38), 12251-12258. http://doi.org/10.1021/bi961153v

Zawadzke, L.E., Smith, T.J., & Herzberg, O. (1995). An engineered Staphylococcus aureus PC1 beta-lactamase that hydrolyses third-generation cephalosporins. PROTEIN ENGINEERING, 8 (12), 1275-1285. http://doi.org/10.1093/protein/8.12.1275

SMITH, T.J., & FOSTER, S.J. (1995). CHARACTERIZATION OF THE INVOLVEMENT OF 2 COMPENSATORY AUTOLYSINS IN MOTHER CELL-LYSIS DURING SPORULATION OF BACILLUS-SUBTILIS-168. JOURNAL OF BACTERIOLOGY, 177 (13), 3855-3862. http://doi.org/10.1128/jb.177.13.3855-3862.1995

HERZBERG, O., KAPADIA, G., BLANCO, B., SMITH, T.S., & COULSON, A. (1991). STRUCTURAL BASIS FOR THE INACTIVATION OF THE P54 MUTANT OF BETA-LACTAMASE FROM STAPHYLOCOCCUS-AUREUS PC1. BIOCHEMISTRY, 30 (39), 9503-9509. http://doi.org/10.1021/bi00103a017

Conference papers

Al-Ammar, R., Nabok, A., Hashim, A., & Smith, T. (2015). Microcystin-LR produced by bacterial algae: Optical detection and purification of contaminated substances. Sensors and Actuators, B: Chemical, 209, 1070-1076. http://doi.org/10.1016/j.snb.2014.11.063

Al-Ammar, R., Nabok, A., Hashim, A., & Smith, T. (2013). Optical detection of microcystin produced by cyanobacteria. Journal of Physics: Conference Series, 450 (1), 012006. http://doi.org/10.1088/1742-6596/450/1/012006

Al-Shanawa, M., Nabok, A., Hashim, A., Smith, T., & Forder, S. (2013). Detection of γ-radiation and heavy metals using electrochemical bacterial-based sensor. Journal of Physics: Conference Series, 450 (1), 012025. http://doi.org/10.1088/1742-6596/450/1/012025

Gittens, J., Smith, T.J., Akid, R., Wang, H., & Greenfield, D. (2010). Biotic Sol-gel coating for the inhibition of marine corrosion and biofouling. Structural Engineer, 88 (21), 21-23.

Gittens, J.E., Wang, H., Smith, T.J., Akid, R., & Greenfield, D. (2009). Biotic sol-gel coating for the inhibition of corrosion in seawater. ECS transactions, 24 (1), 211-229. http://doi.org/10.1149/1.3453618

Zhou, Y., Smith, T.J., & Rainsford, K.D. (2007). A novel component from celery seed with a bactericidal effect against Helicobacter pylori. ZOONOSES AND PUBLIC HEALTH, 54, 63.

Lou, W.Y., Zong, M.H., & Smith, T.J. (2006). Use of ionic liquids to improve whole-cell biocatalytic asymmetric reduction of acetyltrimethylsilane for efficient synthesis of enantiopure (S)-1-trimethylsilylethanol. Green Chemistry, 8 (2), 147-155. http://doi.org/10.1039/b514999a

Book chapters

Smith, T.J., & Nichol, T. (2018). Engineering soluble methane monooxygenase for biocatalysis. In Kalyuzhnaya, M.G., & Xin-Hui, X. (Eds.) Methane biocatalysis: Paving the way to sustainability. (pp. 153-168). Springer International Publishing: http://doi.org/10.1007/978-3-319-74866-5_10

Jiang, H., Chen, Y., Murrell, J.C., Jiang, P., Zhang, C., Xing, X.H., & Smith, T.J. (2011). Methanotrophs: Multifunctional Bacteria with Promising Applications in Environmental Bioengineering. In Comprehensive Biotechnology, Second Edition. (pp. 249-262). http://doi.org/10.1016/B978-0-08-088504-9.00374-3

Murrell, J.C., & Smith, T.J. (2010). Biochemistry and Molecular Biology of Methane Monooxygenase. In Timmis, K.N. (Ed.) Handbook of Hydrocarbon and Lipid Microbiology. (pp. 1045-1055). Springer: http://doi.org/10.1007/978-3-540-77587-4_73

Smith, T.J., Trotsenko, Y.A., & Murrell, J.C. (2010). Physiology and Biochemistry of the Aerobic Methane Oxidizing Bacteria. In Timmis, K.N. (Ed.) Handbook of Hydrocarbon and Lipid Microbiology. (pp. 765-779). Springer: http://doi.org/10.1007/978-3-540-77587-4_58

Smith, T.J., & Murrell, J.C. (2009). Methanotrophy/methane oxidation. In Schaechter, M. (Ed.) Encyclopedia of microbiology. (pp. 293-298). Elsevier: http://www.elsevierdirect.com/product.jsp?isbn=9780123739391&dmnum=CWS1

Books

Smith, T.J., & Dalton, H. (2004). Biocatalysis by methane monooxygenase and its implications for the petroleum industry. http://doi.org/10.1016/s0167-2991(04)80147-2

Theses / Dissertations

Eswyah, A.S. (2018). Bioremediation of selenium species in solution bymethanotrophic bacteria. (Doctoral thesis). Supervised by Gardiner, P. http://doi.org/10.7190/shu-thesis-00070

Codjoe, F.S. (2016). Detection and characterisation of carbapenem-resistant gram-negative bacilli infections in Ghana. (Doctoral thesis). Supervised by Miller, K., & Smith, T.

Al-Luaibi, Y.Y.Y. (2015). Molecular genetics and microbiology of bioremediation using methane-oxidising bacteria. (Doctoral thesis). Supervised by Smith, T., & Stanley, K.

Al-Shanawa, M. (2015). Detection of environmental pollution (radionuclides and heavy metals) using microorganisms. (Doctoral thesis). Supervised by Nabok, A., Hashim, A., Smith, T., & Forder, S.

Challa, S. (2015). Metal contamination and methane oxidising bacteria around a formerly industrialised suburban river. (Doctoral thesis). Supervised by Smith, T.

Ismael, M.M. (2014). Bioremediation of hexavalent chromium using gram-negative bacteria. (Doctoral thesis). Supervised by Smith, T., & Gardiner, P.

Bradshaw, C.E. (2013). Molecular microbial ecology of hospital ward environments. (Doctoral thesis). Supervised by Smith, T., & Stanley, K.

Omar, S.A.S. (2013). Characterisation and bioremediation of soil impacted by Libyan oilfield produced water. (Doctoral thesis). Supervised by Clench, M., & Smith, T.

Nichol, T. (2011). Mutagenic studies into the catalytic versatility of soluble methane monooxygenase. (Doctoral thesis). Supervised by Smith, T.

Kay, G.L. (2010). Microbial ecology and antibiotic resistance of microorganisms in intensive care unit environments. (Doctoral thesis). Supervised by Smith, T., Stanley, K., & Mills, G.

Hamad, B.M.D. (2009). Studies on the biotransformation of chromium (VI) by methane oxidising bacteria. (Doctoral thesis). Supervised by Gardiner, P., & Smith, T.

Zhou, Y. (2008). An antimicrobial agent from celery seed active against H .pylori. (Doctoral thesis). Supervised by Rainsford, K., & Smith, T.

Gittens, J. (n.d.). corrosion. (Doctoral thesis). Supervised by Smith, T.

Postgraduate supervision

Hania Aween - Investigating the molecular basis of bioremediation by methane oxidising bacteria

Salaheldeen Enbaia - Bioremediation of heavy metal pollution using methane oxidising bacteria

Sophie Hutchinson - Investigation of plant products for the development of new antimicrobial drugs



Previous supervision:

Yasin Al-Luaibi - Molecular Genetics and Microbiology of Bioremediation using Methane-Oxidising Bacteria

Mariam Ismael - Bioremediation of Metals and Metalloids by Environmental Bacteria

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