Dr Neil Cross is a Reader in Cancer Biology at Sheffield Hallam University. He completed a BSc in Biomedical Chemistry at Sheffield Hallam University prior to undertaking a PhD and several post-doctoral research positions in cancer biology at The University of Sheffield.
Neil is course leader for BSc Human Biology and teaches aspects of molecular biology and cellular biology particularly in relation to a disease mechanisms context.
His research interests are interdisciplinary projects focussed around cancer biology, and he uses this research to inform his teaching.
Course leader for BSc Human Biology
Neil is module leader for 1st year Topics in Human Biology module, and 2nd year Advanced Cell Biology, and contributes to teaching at all levels of undergraduate teaching including 1st year Human Reproduction and Development, 2nd year Biomedical Investigative Techniques and 3rd year Cell Pathology and Infection. At post-graduate level, he is module leader for Cellular and Molecular Basis of Cancer, and Molecular Diagnostics. He also supervises research projects at undergraduate and post-graduate level.
1) 3D cell culture models
Recent studies funded by NC3Rs and InnovateUK have focused on the development of in vitro alternatives to animal testing using 3D cell culture models. These 3D tumour spheroids mimic many of the biochemical features of tumours such as hypoxia, attenuated drug delivery and low glucose and growth factor levels. Previous work for NC3Rs focused on performing MALDI Mass Spectrometry imaging of small molecules and drugs in these 3D cultures from both cancer, and artificial skin models. Work funded by InnovateUK in collaboration with Asterand Inc. involves developing improved in vitro testing methodologies which may replace patient-derived xenograft/animal testing, by culturing primary human cancer cells in 3D cell culture, allowing drug sensitivity testing to be performed on tumour tissue. This work is in collaboration with Dr Graham Place, Asterand Inc. Related studies with Prof. Malcolm Clench at SHU are using these 3D cell culture models to validate novel methods of quantification of Mass Spectrometry Imaging of proteins within in vitro tissue models.
2) Enhancement of Death Receptor-mediated apoptosis
Recent studies have focused on how tumours evade apoptotic signals from Tumour Necrosis Factor (TNF) superfamily members, with particular emphasis on the potential anti-tumour agent TRAIL (TNF-Related Apoptosis Inducing Ligand). Current studies are focusing on mechanisms of TRAIL resistance in cancer cells, and whether combinations of agents such as histone deactylase (HDAC) inhibitors, nuclear export inhibitors and proteasome inhibitors can reverse TRAIL resistance. In our recent studies, we have also demonstrated that both Histone methytransferases and nuclear export inhibitors in particular can potently enhance tumour cell sensitivity to TRAIL in a range of tumour cells, and that these effects are more potent in 3D cell culture. This work is in collaboration with Dr Andy Chantry, University of Sheffield.
3) Role of cancer stem cells in tumour development and drug resistance
Recent evidence suggests that tumours comprise of differentiated tumour cells, which have a limited life-span, and cancer stem cells/tumour initiating cells, which are undifferentiated cells with unlimited replicative ability and the ability to differentiate. The cancer stem cells hypothesis suggests that if cancer stem cells are selectively ablated, tumour growth will ceases and eventually the tumour will regress. In our studies, we have identified a quiescent/non-dividing population which are intrinsically drug-insensitive and may mediate drug resistance. We are investigating the origin and clinical relevance of these cells in terms of mediating drug resistance and potentially disease metastasis. This work is in collaboration with Dr Colby Eaton and Dr Ning Wang, University of Sheffield.
Ongoing studies in Uveal melanoma are investigating the phenomenon of phenotypic plasticity in terms of reversible differentiation/dedifferentiation which has direct links to the cancer stem cell hypothesis. These studies are in collaboration with Dr Karen Sisley at University of Sheffield.
Furthermore, our studies in breast and prostate stem cell-like cells have demonstrated potent anti-tumour activity of a series of Withanolide-derived compounds which closely resemble androgen-precursors, and these show activity in hormone-dependent cancer cell lines. This work is in collaboration with Dr Tom Sayers, National Institutes of Health, USA.
4) Mitochondrial targeting of anti-tumour agents
The mitochondria are a potential target for anti-tumour agents due to a) their central role in energy dependency and b) their role in regulating apoptosis. Cancer cells have an increased mitochondrial membrane potential vs. normal cells, allowing their selective targeting by lipophilic cations. We have used phosphonium-based conjugation to allow targeting of molecules to the mitochondrion, initially focusing on gold nanoparticles as a cargo. This allows photo-thermal therapy via exposure of specific wavelengths of light, which excite the gold nanoparticles, resulting in mitochondrial release of pro-apoptotic factors. Proof of principle experiments show that a) gold nanoparticles is concentrated in the mitochondria and b) photothermal therapy is enhances in the presence of phosphonium-conjugated gold nanoparticles. This work is in collaboration with Prof. Neil Bricklebank at Sheffield Hallam University.
- Dr Andy Chantry, Mellanby Centre for Bone Biology, University of Sheffield
- Dr Graham Place, Asterand Inc, Royston, UK
- Dr Tom Sayers, National Cancer Institute, National Institutes of Health, USA
- Dr Colby Eaton and Dr Ning Wang, Academic Urology Unit, University of Sheffield
- Dr Karen Sisley, Department of Ophthalmology and Orthoptics, University of Sheffield Medical School
- National Centre for Reduction, Refinement and Replacement of Animals in Research (Nc3Rs)
- Yorkshire Cancer Research
- Bone Cancer Research Trust
- Weston Park Hospital Cancer Appeal
- International Centre for Studies and Research in Biomedicine (ICB)
- Biomolecular Research Centre, Sheffield Hallam University
Spencer, C.E., Flint, L.E., Duckett, C.J., Cole, L.M., Cross, N., Smith, D.P., & Clench, M.R. (2021). Role of MALDI-MSI in Combination with 3D Tissue Models for Early Stage Efficacy and Safety Testing of Drugs and Toxicants. Expert Rev Proteomics. http://doi.org/10.1080/14789450.2021.1876568
Hudson, K., Cross, N., Jordan-Mahy, N., & Leyland, R. (2020). The Extrinsic and Intrinsic Roles of PD-L1 and Its Receptor PD-1: Implications for Immunotherapy Treatment. Frontiers in Immunology, 11, 568931. http://doi.org/10.3389/fimmu.2020.568931
Flint, L.E., Hamm, G., Ready, J.D., Ling, S., Duckett, C.J., Cross, N.A., ... Clench, M.R. (2020). Characterization of an Aggregated Three-Dimensional Cell Culture Model by Multimodal Mass Spectrometry Imaging. Analytical Chemistry. http://doi.org/10.1021/acs.analchem.0c02389
Palubeckaitė, I., Crooks, L., Smith, D., Cole, L., Bram, H., Le Maitre, C., ... Cross, N.A. (2019). Mass spectrometry imaging of endogenous metabolites in response to doxorubicin in a novel 3D osteosarcoma cell culture model. Journal of Mass Spectrometry. http://doi.org/10.1002/jms.4461
Phillips, K., Wright, N., McDermott, E., & Cross, N. (2019). TRAIL responses are enhanced by nuclear export inhibition in osteosarcoma. Biochemical and Biophysical Research Communications, 517 (2), 383-389. http://doi.org/10.1016/j.bbrc.2019.07.047
Mahbub, A.A., Le Maitre, C., Haywood-Small, S., Cross, N., & Jordan-Mahy, N. (2019). Polyphenols enhance the activity of alkylating agents in leukaemia cell lines. Oncotarget, 10 (44). http://doi.org/10.18632/oncotarget.27068
Lalwani, N., Allen, D., Horton, P.N., Coles, S.J., Cross, N., & Bricklebank, N. (2019). Methoxy-phenyl groups reduce the cytotoxicity and increase the aqueous solubility of phosphonium zwitterions and salts. Polyhedron, 158, 515-523. http://doi.org/10.1016/j.poly.2018.10.069
Palubeckaite, I. (2018). Analysis of three dimensional cell cultures using mass spectrometry imaging. (Doctoral thesis). Supervised by Cross, N. http://doi.org/10.7190/shu-thesis-00179
Doherty, R.E., Sisley, K., Hammond, D.W., Rennie, I.G., & Cross, N. (2017). Phenotypic Plasticity in Uveal Melanoma Is Not Restricted to a Tumor Subpopulation and Is Unrelated to Cancer Stem Cell Characteristics. Investigative Opthalmology & Visual Science, 58 (12), 5387. http://doi.org/10.1167/iovs.17-22272
Arhoma, A., Chantry, A.D., Haywood-Small, S., & Cross, N. (2017). SAHA-induced TRAIL-sensitisation of Multiple Myeloma cells is enhanced in 3D cell culture. Experimental cell research. http://doi.org/10.1016/j.yexcr.2017.09.012
Mahbub, A., Le Maitre, C., Haywood-Small, S., Cross, N., & Jordan-Mahy, N. (2017). Dietary polyphenols influence antimetabolite agents: methotrexate, 6-mercaptopurine and 5-fluorouracil in leukemia cell lines. Oncotarget, 8 (62), 104877-104893. http://doi.org/10.18632/oncotarget.20501
Arhoma, A.A. (2017). Enhancement of death receptor-mediated apoptosis in multiple myeloma cells. (Doctoral thesis). Supervised by Cross, N. http://doi.org/10.7190/shu-thesis-00026
Chen, Y.-.S., Allen, D., Cross, N.A., Pitak, M.B., Tizzard, G.J., Coles, S.J., & Bricklebank, N. (2017). Biological and structural studies of phosphonium 'masked thiolate' compounds. European Journal of Medicinal Chemistry, 125, 528-537. http://doi.org/10.1016/j.ejmech.2016.08.025
Wright, N. (2016). Role of cancer stem cells in breast and prostate cancer. (Doctoral thesis). Supervised by Cross, N.
Khalid, H. (2016). PAD4 inhibitors: potential sensitizers of tumour cells to TRAIL-induced apoptosis. Bioscience Horizons, 9, hzw003. http://doi.org/10.1093/biohorizons/hzw003
Harvey, A., Day, R., Cole, L.M., Bartlett, M., Warwick, J., Bojar, R., ... Clench, M.R. (2016). MALDI-MSI for the analysis of a 3D tissue-engineered psoriatic skin model. Proteomics, 16 (11-12), 1718-1725. http://doi.org/10.1002/pmic.201600036
Lalwani, N., Chen, Y.-.S., Brooke, G., Cross, N.A., Allen, D.W., Reynolds, A., ... Bricklebank, N. (2015). Triphenylarsonium-functionalised gold nanoparticles: potential nanocarriers for intracellular therapeutics. Chemical Communications, 51 (19), 4109-4111. http://doi.org/10.1039/C4CC09304F
Mahbub, A.A., Le Maitre, C.L., Haywood-Small, S.L., Cross, N.A., & Jordan-Mahy, N. (2015). Glutathione is key to the synergistic enhancement of doxorubicin and etoposide by polyphenols in leukaemia cell lines. Cell Death and Disease, 6, e2028. http://doi.org/10.1038/cddis.2015.379
Mahbub, A.A.-.H. (2015). The anti-cancer potential of polyphenols in the treatment of leukaemia. (Doctoral thesis). Supervised by Jordan-Mahy, N., Cross, N., Le Maitre, C., & Haywood-Small, S.
Mahbub, A.A., Le Maitre, C.L., Haywood-Small, S.L., Cross, N.A., & Jordan-Mahy, N. (2015). Polyphenols act synergistically with doxorubicin and etoposide in leukaemia cell lines. Cell Death Discovery, 1 (15043), 1-12. http://doi.org/10.1038/cddiscovery.2015.43
Zaini, R., Haywood-Small, S., Cross, N., & Le Maitre, C. (2015). Differential interactions of Falcarinol combined with anti-tumour agents on cellular proliferation and apoptosis in human lymphoid leukaemia cell lines. Journal of Blood Disorders and Transfusion, 6 (2). http://doi.org/10.4172/2155-9864.1000258
Chen, Y.-.S. (2014). Phosphonium-functionalised gold nanoparticles for mitochondria targeted therapeutics. (Doctoral thesis). Supervised by Bricklebank, N., & Cross, N.
Sayers, T., & Cross, N.A. (2014). ISBN-13: 9780199676866. In Rees, R.C. (Ed.) Tumour Immunology and Immunotherapy Chapter 8: Triggering death receptors as a means of inducing tumoricidal activity. Oxford University Press
Sayers, T., & Cross, N.A. (2014). ISBN-13: 9780199676866. In Rees, R.C. (Ed.) Tumour Immunology and Immunotherapy Chapter 8: Triggering death receptors as a means of inducing tumoricidal activity. Oxford University Press
Mahbub, A.A., Le Maitre, C., Haywood-Small, S., McDougall, G.J., Cross, N., & Jordan-Mahy, N. (2013). Differential effects of polyphenols on proliferation and apoptosis in human myeloid and lymphoid leukemia cell lines. Anti-cancer agents in medicinal chemistry, 13 (10), 1601-1613. http://doi.org/10.2174/18715206113139990303
Hsu, Y.P., Staton, C.A., Cross, N., & Buttle, D.J. (2012). Anti-angiogenic properties of ADAMTS-4 in vitro. International Journal Of Experimental Pathology, 93 (1), 70-77. http://doi.org/10.1111/j.1365-2613.2011.00802.x
Ju-Nam, Y., Chen, Y.-.S., Ojeda, J.J., Allen, D.W., Cross, N.A., Gardiner, P.H.E., & Bricklebank, N. (2012). Water-soluble gold nanoparticles stabilized with cationic phosphonium thiolate ligands. RSC Advances, 2 (27), 10345-10351. http://doi.org/10.1039/C2RA21421K
Singh Mudhar, H.S., Scott, I., Ul-Hassan, A., Burton, D., Doherty, R., Cross, N., ... Sisley, K. (2012). Bilateral diffuse uveal melanocytic hyperplasia (BDUMP)-molecular characterisation and novel association with bilateral renal papillary carcinoma. Histopathology, 61 (4), 751-754. http://doi.org/10.1111/j.1365-2559.2011.04171.x
Doherty, R.E., Haywood-Small, S.L., Sisley, K., & Cross, N.A. (2011). Aldehyde dehydrogenase activity selects for the holoclone phenotype in prostate cancer cells. Biochemical and Biophysical Research Communications, 414 (4), 801-807. http://doi.org/10.1016/j.bbrc.2011.10.010
Muthana, M., Giannoudis, A., Scott, S.D., Fang, H.-.Y., Coffelt, S.B., Morrow, F.J., ... Maitland, N.J. (2011). Use of Macrophages to Target Therapeutic Adenovirus to Human Prostate Tumors. Cancer Research, 71, 1805-1815. http://doi.org/10.1158/0008-5472.CAN-10-2349
Kokab, A., Jennings, R., Eley, A., Pacey, A.A., & Cross, N.A. (2010). Analysis of modulated gene expression in a model of Interferon-gamma-induced persistence of Chlamydia trachomatis in HEp-2 cells. Microbial Pathogenesis, 49 (5), 217-225. http://doi.org/10.1016/j.micpath.2010.06.002
Guzmán-Ramírez, N., Völler, M., Wetterwald, A., Germann, M., Cross, N.A., Rentsch, C.A., ... Cecchini, M.G. (2009). In vitro propagation and characterization of neoplastic stem/progenitor-like cells from human prostate cancer tissue. The Prostate, 69 (15), 1683-1693. http://doi.org/10.1002/pros.21018
Al-Mously, N., Cross, N.A., Eley, A., & Pacey, A.A. (2009). Real-time polymerase chain reaction shows that density centrifugation does not always remove Chlamydia trachomatis from human semen. Fertility and sterility, 92 (5), 1606-1615. http://doi.org/10.1016/j.fertnstert.2008.08.128
Cross, N., Waterman, E.A., Jokonya, N., Fowles, A., Buckle, C.H., Phillips, J., ... Eaton, C.L. (2008). Phenotypic variations of TRAIL sensitivity in cloned populations of prostate cancer cells. Journal of cellular biochemistry, 104 (4), 1452-1464. http://doi.org/10.1002/jcb.21721
Cross, N.A., Fowles, A., Reeves, K., Jokonya, N., Linton, K., Holen, I., ... Eaton, C.L. (2008). Imaging the effects of castration on bone turnover and hormone-independent prostate cancer colonization of bone. The Prostate, 68 (15), 1707-1714. http://doi.org/10.1002/pros.20833
Tozer, G.M., Akerman, S., Cross, N.A., Barber, P.R., Bjorndahl, M.A., Greco, O., ... Kanthou, C. (2008). Blood vessel maturation and response to vascular-disrupting therapy in single vascular endothelial growth factor-A isoform-producing tumors. Cancer research, 68 (7), 2301-2311. http://doi.org/10.1158/0008-5472.CAN-07-2011
Waterman, E.A., Cross, N.A., Lippitt, J.M., Cross, S.S., Rehman, I., Holen, I., ... Eaton, C.L. (2007). The antibody MAB8051 directed against osteoprotegerin detects carbonic anhydrase II: Implications for association studies with human cancers. International Journal of Cancer, 121 (9), 1958-1966. http://doi.org/10.1002/ijc.22946
Cross, N.A., Papageorgiou, M., & Eaton, C.L. (2007). Bone marrow stromal cells promote growth and survival of prostate cancer cells. Biochemical Society Transactions, 35 (Pt 4), 698-700.
Bryant, R.J., Cross, N.A., Eaton, C.L., Hamdy, F.C., & Cunliffe, V.T. (2007). EZH2 promotes proliferation and invasiveness of prostate cancer cells. The Prostate, 67 (5), 547-556. http://doi.org/10.1002/pros.20550
Cross, N.A., Reid, S.V., Harvey, A.J., Jokonya, N., & Eaton, C.L. (2006). Opposing actions of TGFbeta1 and FGF2 on growth, differentiation and extracellular matrix accumulation in prostatic stromal cells. Growth Factors, 24 (4), 233-241. http://doi.org/10.1080/08977190600976501
Cross, N.A., Ganesh, A., Parpia, M., Murray, A.K., Rennie, I.G., & Sisley, K. (2006). Multiple locations on chromosome 3 are the targets of specific deletions in uveal melanoma. Eye, 20 (4), 476-81. http://doi.org/10.1038/sj.eye.6701906
Holen, I., Cross, S.S., Neville-Webbe, H.L., Cross, N.A., Balasubramanian, S.P., Croucher, P.I., ... Eaton, C.L. (2005). Osteoprotegerin (OPG) expression by breast cancer cells in vitro and breast tumours in vivo - a role in tumour cell survival? Breast Cancer Research and Treatment, 92 (3), 207-15. http://doi.org/10.1007/s10549-005-2419-8
Cross, N.A., Chandrasekharan, S., Jokonya, N., Fowles, A., Hamdy, F.C., Buttle, D.J., & Eaton, C.L. (2005). The expression and regulation of ADAMTS-1, -4, -5, -9, and -15, and TIMP-3 by TGFbeta1 in prostate cells: relevance to the accumulation of versican. The Prostate, 63 (3), 269-75. http://doi.org/10.1002/pros.20182
Cross, N.A., Rennie, I.G., Murray, A.K., & Sisley, K. (2005). The identification of chromosome abnormalities associated with the invasive phenotype of uveal melanoma in vitro. Clinical & Experimental Metastasis, 22 (2), 107-113. http://doi.org/10.1007/s10585-005-5142-2
Nyambo, R., Cross, N., Lippitt, J., Holen, I., Bryden, G., Hamdy, F.C., & Eaton, C.L. (2004). Human bone marrow stromal cells protect prostate cancer cells from TRAIL-induced apoptosis. Journal of Bone and Mineral Research (JBMR), 19 (10), 1712-1721. http://doi.org/10.1359/JBMR.040703
Neville-Webbe, H.L., Cross, N.A., Eaton, C.L., Nyambo, R., Evans, C.A., Coleman, R.E., & Holen, I. (2004). Osteoprotegerin (OPG) produced by bone marrow stromal cells protects breast cancer cells from TRAIL-induced apoptosis. Breast Cancer Research and Treatment, 86 (3), 269-279. http://doi.org/10.1023/B:BREA.0000036900.48763.b3
Dizeyi, N., Bjartell, A., Nilsson, E., Hansson, J., Gadaleanu, V., Cross, N., & Abrahamsson, P.-.A. (2004). Expression of serotonin receptors and role of serotonin in human prostate cancer tissue and cell lines. The Prostate, 59 (3), 328-336. http://doi.org/10.1002/pros.10374
Cross, N.A., Murray, A.K., Rennie, I.G., Ganesh, A., & Sisley, K. (2003). Instability of microsatellites is an infrequent event in uveal melanoma. Melanoma Research, 13 (5), 435-440.
Woodward, J.K.L., Elshaw, S.R., Murray, A.K., Nichols, C.E., Cross, N., Laws, D., ... Sisley, K. (2002). Stimulation and inhibition of uveal melanoma invasion by HGF, GRO, IL-1alpha and TGF-beta. Investigative ophthalmology & visual science, 43 (10), 3144-3152.
Elshaw, S.R., Sisley, K., Cross, N., Murray, A.K., MacNeil, S.M., Wagner, M., ... Rennie, I.G. (2001). A comparison of ocular melanocyte and uveal melanoma cell invasion and the implication of α1β1, α4β1 and α6β1 integrins. British Journal of Ophthalmology, 85 (6), 732-738. http://doi.org/10.1136/bjo.85.6.732
Cross, N.A., Kellock, D.J., Kinghorn, G.R., Taraktchoglou, M., Bataki, E., Oxley, K.M., ... Eley, A. (1999). Antimicrobial susceptibility testing of Chlamydia trachomatis using a reverse transcriptase PCR-based method. Antimicrobial agents and chemotherapy, 43 (9), 2311-2313. http://aac.asm.org/content/43/9/2311
Brooks, A.D., Wright, N.E., Xu, Y.-.M., Wijeratne, K., Tewary, P., Cross, N., ... Sayers, T.J. (2018). 17beta-hydroxywithanolides inhibit the proliferation of castration-resistant prostate cancer cells by reducing levels of cFLIP. CANCER RESEARCH, 78 (13). http://doi.org/10.1158/1538-7445.AM2018-2671
Harvey, A.C., Place, G., Baggott, K., Samra, S., Freathy, C., & Cross, N. (2017). Development of robust in vitro 3D models of human tumors for the identification and evaluation of anticancer drugs. CANCER RESEARCH, 77 (22). http://doi.org/10.1158/1538-7445.NEWFRONT17-B30
Harvey, A., Cole, L., Clench, M., Cross, N., & Smith, D. (2015). MALDI-IMS-MSI for the Analysis of 3D Tissue-Engineered Psoriatic Skin Models. TISSUE ENGINEERING PART A, 21, S163.
Zaini, R., Small, S.H., Cross, N., & Le Maitre, C. (2015). Combination therapy with Falcarinol on human lymphoid leukaemia cell lines and increase the expression of cytochrome C, Bax and SMAC/Diablo. BRITISH JOURNAL OF HAEMATOLOGY, 169, 72.
Doherty, R., Hoh, L., Rennie, I., Cross, N., & Sisley, K. (2012). Isolation and characterisation of cancer stem cells in uveal melanoma. INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE, 30, S61.
Wilson, A., Jordan-Mahy, N., Haywood-Small, S., Cross, N.A., & Le Maitre, C. (2012). Extracts From Red and White Cabbage Contains Bio-Active Compounds Which Induce Apoptosis in Myeloid and Lymphoid Leukaemia Cell Lines. JOURNAL OF PATHOLOGY, 228, S37.
Mahbub, A., Le Maitre, C., Haywood-Small, S.L., McDougall, G., Cross, N.A., & Jordan-Mahy, N. (2012). The Anti-cancerous Potential of Polyphenols in the Treatment of Human Myeloid and Lymphoid Leukaemia. JOURNAL OF PATHOLOGY, 228, S34.
Zaini, R., Haywood, S., Brandt, K., Clench, M.R., Cross, N., & Le Maitre, C. (2012). Synergistic Action of Falcarinol on Induction of Apoptosis on Human Lymphoid Leukaemia Cell Lines. JOURNAL OF PATHOLOGY, 228, S38.
Doherty, R.E., Hoh, L., Rennie, I., Sisley, K., & Cross, N.A. (2012). Isolation and Characterisation of Cancer Stem Cells in Solid Tumours. JOURNAL OF PATHOLOGY, 228, S34.
Doherty, R., Hoh, L., Rennie, I., Sisley, K., & Cross, N. (2012). Isolation and Characterisation of Cancer Stem Cells in Solid Tumours. EUROPEAN JOURNAL OF CANCER, 48, S65-S66. http://doi.org/10.1016/S0959-8049(12)70964-4
Adeniji, O.O., Molokwu, C.N., Waterman, E.A., Cross, N.A., Hamdy, F.C., & Buttle, D.J. (2008). TIMP-3 expression is regulated by androgen and TNF in prostate stromal and cancer cells. INTERNATIONAL JOURNAL OF EXPERIMENTAL PATHOLOGY, 89 (3), A9.
Adeniji, O.O., Molokwu, C.N., Waterman, E.A., Cross, N.A., Hamdy, F.C., & Buttle, D.J. (2007). TIMP-3 expression in prostate stromal and tumour cells is regulated by androgen and TNF. INTERNATIONAL JOURNAL OF EXPERIMENTAL PATHOLOGY, 88 (6), A93-A94.
Woodward, J., Lefley, D., Cross, N., Ottewell, P.D., Buttle, D.J., Coleman, R.E., & Holen, I. (2007). Tumour cell-bone marrow stromal cell interactions modify expression of cathepsin K, ADAMTS-15, TIMP-3 and osteoprotegerin (OPG). INTERNATIONAL JOURNAL OF EXPERIMENTAL PATHOLOGY, 88 (6), A97.
Cross, N.A., Papageorgiou, M., Lippitt, J., Nyambo, R., Hamdy, F.C., & Eaton, C.L. (2007). Bone marrow stromal cell-derived insulin-like growth factor (IGF) II enhances growth and survival of prostate cancer cells and potentiates androgen action. INTERNATIONAL JOURNAL OF EXPERIMENTAL PATHOLOGY, 88 (6), A94.
Adeniji, O.O., Molokwu, C.N., Waterman, E.A., Cross, N.A., Hamdy, F.C., & Buttle, D.J. (2007). Evaluating the role of ADAMTS proteoglycanases and TIMP-3 in prostate cancer progression. INTERNATIONAL JOURNAL OF EXPERIMENTAL PATHOLOGY, 88 (4), A58.
Woodward, J.K.L., Lefley, D.V., Cross, N.A., Ottewell, P.D., Battle, D.J., Coleman, R.E., & Holen, I. (2007). Tumour cell-bone marrow stromal cell interactions modify expression of cathepsin K, ADAMTS-15, TIMP-3 and osteoprotegerin (OPG). JOURNAL OF BONE AND MINERAL RESEARCH, 22 (7), 1142-1143.
Phillips, J., Rissanen, M., Cross, N., Proctor, L., Sokhi, D., Vaisanen, V., ... Hamdy, F.C. (2007). Qualitative and quantitative PSA mRNA detection in prostate cancer - A useful tool for the clinician? EUROPEAN UROLOGY SUPPLEMENTS, 6 (2), 250. http://doi.org/10.1016/S1569-9056(07)60904-X
Bryant, R.J., Cross, N.A., Eaton, C.L., Hamdy, F.C., & Cunliffe, V.T. (2007). The polycomb group protein EZH2 is required for transcriptional repression of p21 and MMP7 in prostate cancer cells. EUROPEAN UROLOGY SUPPLEMENTS, 6 (2), 131. http://doi.org/10.1016/S1569-9056(07)60433-3
Papageorgiou, M., Lippitt, J., Nyambo, R., Cross, N., Hamdy, F.C., & Eaton, C.L. (2005). Human bone marrow stromal cell derived IGF signaling enhances PSA production as well as proliferation/survival of androgen sensitive prostate cancer cells in vitro. CANCER TREATMENT REVIEWS, 31, S36.
Molokwu, C., Waterman, E., Cross, N., Buttle, D.J., Hamdy, F.C., & Eaton, C. (2005). Analysis of the functional role of ADAMTS enzymes in prostate cancer. BJU INTERNATIONAL, 95, 29.
Nyambo, R., Neville-Webbe, H., Holen, I., Cross, N.A., Hamdy, F.C., & Eaton, C.L. (2004). Human bone marrow stromal cells protect prostate and breast cancer cells from TRAIL-induced apoptosis. JOURNAL OF BONE AND MINERAL RESEARCH, 19 (9), 1572.
Nyambo, R., Cross, N.A., Lippit, J., Holen, I., Bryden, A.A.G., & Eaton, C.L. (2003). Human bone marrow stromal cells (hBMSC) protect prostate cancer cells from trail induced apoptosis. BRITISH JOURNAL OF CANCER, 88, S26.
Chandrasekharan, S., Buttle, D.J., Holen, I., Cross, N.A., & Hamdy, F.C. (2003). Expression pattern of the ADAMTS (A Disintegrin And Metalloproteinase with ThromboSpondin motifs) proteinases in human prostate cancer celllines. JOURNAL OF UROLOGY, 169 (4), 58.
Cross, N., Harvey, A., & Eaton, C.L. (2002). Autocrine activities of transforming growth factor beta 1 in prostatic stromal cells. JOURNAL OF UROLOGY, 167 (4), 220-221.
Hudson, K., Cross, N., Jordan-Mahy, N., & Leyland, R. (2020). Blockade of Programmed Death-Ligand 1 with Atezolizumab in Human Breast Cancer 3D Spheroid Colonies Induces Changes in Cell Viability. Presented at: EACR-AACR-ASPIC Conference: Tumour Microenvironment, Lisbon, Portugal, 2020
Hudson, K., Jordan-Mahy, N., Cross, N., & Leyland, R. (2019). Programmed death-ligand 1 expression in human cancer cell lines in two-dimensional and three-dimensional cell culture systems. Presented at: BACR: Tumour Microenvironment meeting, Nottingham, UK
Stokes, H., Cross, N., & Leyland, R. (2018). Characterisation of novel lung cancer cell lines for immuno-inhibitory markers. Presented at: BMRC/MERI Winter Poster Event 2018, Sheffield, 2018
Hudson, K., Cross, N., Jordan-mahy, N., & Leyland, R. (2018). Flow cytometric phenotyping of diverse human cancer cell lines for immunological biomarkers expression. Presented at: BMRC/MERI Winter Poster Event, Sheffield, 2018
Dowling, J., Ferriera de Matos, C., Leyland, R., & Cross, N. (2018). Investigation of TRAIL resistance in lung cancer cell lines. Presented at: British Society for Immunology: Yorkshire Immunology Group Symposium 2018, University of York, UK, 2018
Dr Cross is a member of the University validation panels, assessing new course provision at course approval events. He is also a specialist scientific reviewer for InnovateUK.
- Ieva Palubeckaite - Analysis of three dimensional cell cultures using mass spectrometry imaging
- Imran Jabbar - Modelling Human Chorionic Gonadotropin Immunoassay for Treatment Response in Low-Risk Gestational Trophoblastic Neoplasia
- Lucy Flint - Biopharmaceutical Quantification by Mass Spectrometry Imaging
Previous PhD students:
- Amal Arhoma - Enhancement of Death receptor-mediated apoptosis on Multiple Myeloma
- Nikhil Lalwani - Triphenylarsonium-functionalised gold nanoparticles: Potential nanocarriers for intracellular therapeutics.
- Nicola Wright - Role of cancer stem cells in breast and prostate cancer.
- Amani Mahbub - The anti-cancer potentials of polyphenols in the treatment of leukemia.
- Jenni Chen -Phosphonium-functionalised gold nanoparticles for mitochondrial-targeted therapeutics
- Rachel Doherty - The role of Cancer Stem Cells in uveal melanoma.
- Abas Kokab - The role of Chlamydia trachomatis in male infertility.